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Changes in lncRNAs and related genes in β-thalassemia minor and β-thalassemia major
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《医学前沿(英文)》 2017年 第11卷 第1期 页码 74-86 doi: 10.1007/s11684-017-0503-1
β-thalassemia is caused by β-globin gene mutations. However, heterogeneous phenotypes were found in individuals with same genotype, and still undescribed mechanism underlies such variation. We collected blood samples from 30 β-thalassemia major, 30 β-thalassemia minor patients, and 30 matched normal controls. Human lncRNA Array v2.0 (8 × 60 K, Arraystar) was used to detect changes in long non-coding RNAs (lncRNAs) and mRNAs in three samples each from β-thalassemia major, β-thalassemia minor, and control groups. Compared with normal controls, 1424 and 2045 lncRNAs were up- and downregulated, respectively, in β-thalassemia major patients, whereas 623 and 349 lncRNAs were up- and downregulated, respectively, in β-thalassemia minor patients. Compared with β-thalassemia minor group, 1367 and 2356 lncRNAs were up- and downregulated, respectively, in β-thalassemia major group. We selected five lncRNAs that displayed altered expressions (DQ583499, X-inactive specific transcript (Xist), lincRNA-TPM1, MRFS16P, and lincRNA-RUNX2-2) and confirmed their expression levels in all samples using real-time polymerase chain reaction. Based on coding-non-coding gene co-expression network and gene ontology biological process analyses, several signaling pathways were associated with three common organ systems exhibiting β-thalassemia phenotypes: hematologic, skeletal, and hepatic systems. This study implicates that abnormal expression levels of lncRNAs and mRNA in β-thalassemia cases may be correlated with its various clinical phenotypes.
关键词: β-thalassemia long non-coding RNA mRNA phenotypic heterogeneity pathway
Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, Guoying Huang
《医学前沿(英文)》 2021年 第15卷 第1期 页码 91-100 doi: 10.1007/s11684-020-0778-5
关键词: congenital heart disease Gene Expression Omnibus lncRNA SAP30-2:1 cell proliferation RNA immunoprecipitation HAND2
Xuefei Ma, Wei Zhang, Rong Zhang, Jingming Li, Shufen Li, Yunlin Ma, Wen Jin, Kankan Wang
《医学前沿(英文)》 2019年 第13卷 第3期 页码 330-343 doi: 10.1007/s11684-017-0557-0
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《医学前沿(英文)》 2016年 第10卷 第3期 页码 297-310 doi: 10.1007/s11684-016-0452-0
Studies on coding genes, miRNAs, and lncRNAs during erythroid development have been performed in recent years. However, analysis focusing on the integration of the three RNA types has yet to be done. In the present study, we compared the dynamics of coding genes, miRNA, and lncRNA expression profiles. To explore dynamic changes in erythropoiesis and potential mechanisms that control these changes in the transcriptome level, we took advantage of high throughput sequencing technologies to obtain transcriptome data from cord blood hematopoietic stem cells and the following four erythroid differentiation stages, as well as from mature red blood cells. Results indicated that lncRNAs were promising cell marker candidates for erythroid differentiation. Clustering analysis classified the differentially expressed genes into four subtypes that corresponded to dynamic changes during stemness maintenance, mid-differentiation, and maturation. Integrated analysis revealed that noncoding RNAs potentially participated in controlling blood cell maturation, and especially associated with heme metabolism and responses to oxygen species and DNA damage. These regulatory interactions were displayed in a comprehensive network, thereby inferring correlations between RNAs and their associated functions. These data provided a substantial resource for the study of normal erythropoiesis, which will permit further investigation and understanding of erythroid development and acquired erythroid disorders.
关键词: erythroid differentiation hematopoietic stem cell RNA-seq miRNA lncRNA
Xu-guang ZUO, Lu YU
《信息与电子工程前沿(英文)》 2018年 第19卷 第3期 页码 459-470 doi: 10.1631/FITEE.1601552
Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the
《医学前沿(英文)》 页码 924-938 doi: 10.1007/s11684-023-1004-z
关键词: ovarian cancer lncRNA LOC646029 metastasis microRNA 627-3p SPRED1
Jizu LV, Minli BAI, Long ZHOU, Jian ZHOU,
《能源前沿(英文)》 2010年 第4卷 第3期 页码 392-401 doi: 10.1007/s11708-009-0066-1
关键词: heat transfer space non-uniformity soot emission in-cylinder diesel
Ribozyme and the mechanisms that underlie RNA catalysis
Timothy J. Wilson,Yijin Liu,David M. J. Lilley
《化学科学与工程前沿(英文)》 2016年 第10卷 第2期 页码 178-185 doi: 10.1007/s11705-016-1558-2
QIAO Congzhen, LI Chengyue
《化学科学与工程前沿(英文)》 2008年 第2卷 第1期 页码 69-73 doi: 10.1007/s11705-008-0014-3
关键词: stability different hypecritical compulsive mixing-reacting-separating-recycling non-benzene
REDUCTION OF NON-POINT SOURCE POLLUTION IN THE YONG’AN RIVER BY CONSTRUCTED WETLAND BASED ON 9 YEARS
《农业科学与工程前沿(英文)》 2023年 第10卷 第4期 页码 627-638 doi: 10.15302/J-FASE-2023516
The agricultural and livestock activities surrounding the rivers flowing into the lakes have caused non-point source pollution, leading to excessive amounts of nutrient salts in downstream rivers. Introducing river water into constructed wetlands along river course has proven to be an effective solution for decreasing nitrogen (N) and phosphorus (P) loads. This paper reports 9 years of monitoring the Yong’an River and its surrounding constructed wetlands in the upper reaches of Erhai Lake, located in Yunnan Province, China. This study analyzed the main types of pollutants in the river, and evaluated the removal efficiency of pollutants by the constructed wetlands. The findings indicate that total nitrogen (TN) and nitrate nitrogen (NO3–-N) are the primary pollutants in the Yong’an River, which exhibit variation throughout the year corresponding to the alternating wet and dry seasons. Although constructed wetlands are effective in removing NO3–-N and P, their efficacy in removing ammonium nitrogen (NH4+-N) and organic pollutants is limited. This limitation can be attributed to the lack of timely disposal of aquatic plant residues. This research contributes to the understanding of the potential issues that may arise during the extended use of constructed wetlands and provides solutions to address them.
关键词: inflowing rivers surface-flow constructed wetland nutrients long-term monitoring
基于RNA的生物防治——一种作物保护新模式 Review
Matthew Bramlett, Geert Plaetinck, Peter Maienfisch
《工程(英文)》 2020年 第6卷 第5期 页码 522-527 doi: 10.1016/j.eng.2019.09.008
关键词: 基于RNA的生物防治 RNA干扰(RNAi) 科罗拉多马铃薯甲虫(CPB) 玉米根虫(CRW) 大豆臭虫(SSB)
Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages
LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao
《医学前沿(英文)》 2008年 第2卷 第2期 页码 166-170 doi: 10.1007/s11684-008-0030-1
Research and implementation of flexible coding system oriented multi-view
ZHANG Xuhui, ZHANG Xu, NING Ruxin
《机械工程前沿(英文)》 2007年 第2卷 第4期 页码 448-452 doi: 10.1007/s11465-007-0077-8
标题 作者 时间 类型 操作
Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation
Jing Ma, Shiyu Chen, Lili Hao, Wei Sheng, Weicheng Chen, Xiaojing Ma, Bowen Zhang, Duan Ma, Guoying Huang
期刊论文
Overexpressed long noncoding RNA CRNDE with distinct alternatively spliced isoforms in multiple cancers
Xuefei Ma, Wei Zhang, Rong Zhang, Jingming Li, Shufen Li, Yunlin Ma, Wen Jin, Kankan Wang
期刊论文
Global transcriptome analysis for identification of interactions between coding and noncoding RNAs during
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期刊论文
Long noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the
期刊论文
Effect of heat transfer space non-uniformity of combustion chamber components on in-cylinder heat transfer
Jizu LV, Minli BAI, Long ZHOU, Jian ZHOU,
期刊论文
Ribozyme and the mechanisms that underlie RNA catalysis
Timothy J. Wilson,Yijin Liu,David M. J. Lilley
期刊论文
Continuous reaction performances of benzene alkylation with long chain olefins catalyzed by ionic liquid
QIAO Congzhen, LI Chengyue
期刊论文
REDUCTION OF NON-POINT SOURCE POLLUTION IN THE YONG’AN RIVER BY CONSTRUCTED WETLAND BASED ON 9 YEARS
期刊论文
Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages
LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao
期刊论文